Although the standard of care for this type of cancer is treatment with gemcitabine—the most commonly used chemotherapy in the treatment of pancreatic adenocarcinoma—the CureMatch platform instead recommended a combination of two specific drugs (“Drug A” and “Drug B”)* to simultaneously target the two actionable mutations.
In order to assess the efficacy of the recommended treatment, four scenarios were compared: (1) standard chemotherapy (gemcitabine); (2) personalized monotherapy using Drug A only; (3) personalized monotherapy using Drug B only; and (4) personalized combination therapy using both Drug A and B (the CureMatch-recommended therapy). A graphic representation is shown below, with the two actionable mutations shown on the DNA strands in red and green.
The effects of the drug treatments were measured using a cell viability assay, which measures the number of living cells remaining after treatment. Matched combination therapy (the CureMatch-recommended therapy), at 1:1 concentrations of Drug A:Drug B, not only resulted in a significant increase in cell death compared to the matched monotherapies (3-5 fold increase), but also in a significant increase in cell death when compared to standard chemotherapy (>20-fold increase).
This in vitro data supports the notion that combinations of drugs specifically “matched” to actionable mutations present in a patient’s tumor have the potential to provide greatly improved outcomes compared not only to standard chemotherapy but to matched monotherapy. This the basis of the CureMatch approach.